Low-percentage acrylamide gels are also used to separate DNA. Gels of between 10 and 20% acrylamide are used in techniques such as SDS–gel electrophoresis, where the smaller pore size now introduces a sieving effect that contributes to the separation of proteins according to their size. 

TLS Online TPP Program

#Id: 3593

#Unit 3. Fundamental Processes

The side chains of amino acids have little or no specific affinity for the purine and pyrimidine bases found in RNA.

TLS Online TPP Program

#Id: 3594

#Unit 3. Fundamental Processes

In 1961 Marshall Nirenberg and Heinrich Matthaei reported the first breakthrough in reading nucleotide codon in amino acid frame.

TLS Online TPP Program

#Id: 3596

#Unit 3. Fundamental Processes

Nirenberg and Matthaei therefore concluded that the triplet codon UUU encodes phenylalanine, poly(C) proline (polyproline), polyadenylate, poly(A), encodes polylysine.

TLS Online TPP Program

#Id: 3597

#Unit 3. Fundamental Processes

“Dictionary” of amino acid code words in mRNAs.

TLS Online TPP Program

#Id: 3599

#Unit 3. Fundamental Processes

The third base of each codon plays a lesser role in specifying an amino acid than the first two.

TLS Online TPP Program

#Id: 3600

#Unit 3. Fundamental Processes

All the amino acids except methionine and tryptophan have more than one codon.