Long primers forms most probably dimers, so taq pol amplifying this (in low temp) also.

TLS Online TPP Program

#Id: 802

#Unit 3. Fundamental Processes

According to Baltimore, for transcription antisense (-) strand of genome is required

TLS Online TPP Program

#Id: 803

#Unit 3. Fundamental Processes

There are two factors that regulate Prokaryotic Transcription

1. RNA Polymerase

2. Promoter Location & Sequences

TLS Online TPP Program

#Id: 804

#Unit 3. Fundamental Processes

RNA Polymerase SDS page shows two very large subunits: beta (b) and beta-prime (b’), with molecular masses of 150 and 160 kD, respectively.

TLS Online TPP Program

#Id: 805

#Unit 3. Fundamental Processes

The holoenzyme could transcribe intact phage T4 DNA in vitro quite actively, the core enzyme had little ability to do this.

TLS Online TPP Program

#Id: 806

#Unit 3. Fundamental Processes

Hinkle and Chamberlin experiment showed that the polymerase holoenzyme binds much more tightly to the T7 DNA than does the core enzyme.

TLS Online TPP Program

#Id: 807

#Unit 3. Fundamental Processes

Hinkle and Chamberlin experiment also showed that the melted DNA has higher affinity for holoenzyme