Sometimes the S1 nuclease will not digest the single stranded regions completely, so the transcript appears to be a little longer than it really is, But another method, called primer extension, can tell the 5’-end (but not the 3’-end) to the exact nucleotide

TLS Online TPP Program

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#Unit 6. System Physiology – Plant

Cytochalasin D, an inhibitor of actin polymerization, also inhibits AUXIN efflux

TLS Online TPP Program

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#Unit 6. System Physiology – Plant

Auxin-induced proton extrusion acidifies the cell wall and increases cell extension

Auxin increase the rate of proton extrusion (wall acidification), and the kinetics of proton extrusion should closely match those of auxin-induced growth.

TLS Online TPP Program

#Id: 10137

#Unit 6. System Physiology – Plant

5 Genes involved in auxin-regulated growth and development:

1. The AUX/IAA gene family

2. The SAUR gene family

3. The GH3 gene family

Stress response genes:

1. Genes encoding glutathione S-transferases

2. Genes encoding 1-aminocyclopropane-1-carboxylic

acid (ACC) synthase

TLS Online TPP Program

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#Unit 6. System Physiology – Plant

In the absence of IAA, the Transcription factor, ARF, forms Inactive heterodimers with AUX/IAA proteins.

TLS Online TPP Program

#Id: 10139

#Unit 6. System Physiology – Plant

TLS Online TPP Program

#Id: 10140

#Unit 6. System Physiology – Plant

The auxin receptor is composed of two proteins: the SCF complex component TIR1 and the repressor protein AUX/IAA.