Vectors For The Production Of Transgenic Plants

Plasmid vectors (Ti plasmid, Ri plasmid)

Plant virus vectors (Cauliflower Mosaic Virus, Gemini Viruses, Tobacco Mosaic Virus)

TLS Online TPP Program

#Id: 7934

#Unit 2. Cellular Organization

The two key components in protein targeting in ER:

Signal recognition particle (SRP)

SRP receptor

TLS Online TPP Program

#Id: 7935

#Unit 2. Cellular Organization

The SRP is a cytosolic ribonucleoprotein particle that transiently binds to both the ER signal sequence in a nascent protein and the large (60S) ribosomal subunit, forming a large complex.

TLS Online TPP Program

#Id: 7936

#Unit 2. Cellular Organization

The SRP is made up of six proteins bound to a 300-nucleotide RNA, which acts as a scaffold for the hexamer.

TLS Online TPP Program

#Id: 7937

#Unit 2. Cellular Organization

Once the ER signal sequence emerges from the ribosome, it is bound by a signal recognition particle (SRP)

TLS Online TPP Program

#Id: 7938

#Unit 2. Cellular Organization

The SRP and the nascent polypeptide chain–ribosome complex bind to the SRP receptor in the ER membrane. This interaction is strengthened by the binding of GTP to both the SRP and its receptor.

TLS Online TPP Program

#Id: 7939

#Unit 2. Cellular Organization

The GTPase cycle analogous to the GTP hydrolysis coupled to the initiation of translation on the ribosome.