Damage leads to the proteolytic destruction of a transcriptional repressor (the LexA repressor) that controls expression of genes involved in the SOS response, including those for DinB, UmuC, and UmuD, the inactive precursor for UmuD0.

TLS Online TPP Program

#Id: 2508

#Unit 13. Methods in Biology

Cellulose Acetate Electrophoresis have clinical role for example enzyme and antibodies are easily detected by using Cellulose Acetate Electrophoresis with zymogen technique.

TLS Online TPP Program

#Id: 2509

#Unit 13. Methods in Biology

The most commonly used general protein stain for detecting protein on gels is the sulfated trimethylamine dye Coomassie Brilliant Blue R-250 (CBB).

TLS Online TPP Program

#Id: 2510

#Unit 13. Methods in Biology

Silver staining gives higher sensitivity comparison to Coomassie Brilliant Blue (CBB).

TLS Online TPP Program

#Id: 2511

#Unit 13. Methods in Biology

Glycoproteins have traditionally been detected on protein gels by use of the periodic acid–Schiff ( PAS) stain

TLS Online TPP Program

#Id: 2512

#Unit 13. Methods in Biology

Quantitative analysis (i.e., measurements of the relative amounts of different proteins in a sample) can be achieved by scanning densitometry .

TLS Online TPP Program

#Id: 2513

#Unit 13. Methods in Biology

Larger fragment DNA are isolated by using agarose gel while shorter fragment are separated by 3.5% polyacrylamide gels are used to separate DNA in the range 80–1000 nt and 12% gels to resolve fragments of between 20 and 100 nt.