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Thicker sections must be fixed, dehydrated, embedded in plastic, sectioned, and then stained with electron dense heavy metals for TEM.





#Unit 13. Methods in Biology #Electron Microscopy #Part B Pointers
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#Unit 4. Cell Communication and Cell Signaling

T-Cell Activation and Differentiation

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#Unit 4. Cell Communication and Cell Signaling

Cross-presentation

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#Unit 1. Molecules and their Interaction Relevant to Biology

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#Unit 4. Cell Communication and Cell Signaling

The principal ECM adhesion receptor in epithelial hemidesmosomes is integrin α6β4.

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#Unit 13. Methods in Biology

 If the antibody preparation is polyclonal and has a range of affinities, Scatchard plot yields a curved line whose slope is constantly changing. The average affinity constant K0 can be calculated by determining the value of Ka when half of the binding sites are occupied (i.e., when r  1 in this example). In this graph, antiserum #3 has a higher affinity (K0  2.4  108) than antiserum #4 (K0  1.25  108)


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