SeqA binds to hemimethylated DNA and is required for 13 min delaying rereplication.

TLS Online TPP Program

#Id: 7419

#Unit 5. Developmental Biology

Ultrabithorax (Ubx), is required for the identity of the third thoracic segment.

TLS Online TPP Program

#Id: 2533

#Unit 13. Methods in Biology

The ‘Wallace rule’ (Lay Thein & Wallace 1986) is used to determine the appropriate stringency wash temperature: 

Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs

TLS Online TPP Program

#Id: 3371

#Unit 3. Fundamental Processes

Structural studies show that the active site of DNA Pol h is better at accommodating a thymine dimer than is the active site of another translesion DNA polymerase (DNA Pol k

TLS Online TPP Program

#Id: 2418

#Unit 3. Fundamental Processes

he ability of the enzyme to terminate in vitro, however, is strongly influenced by parameters such as the ionic strength and temperature at which the reaction is performed.

TLS Online TPP Program

#Id: 2637

#Unit 13. Methods in Biology

Primer extension Using primer extension one can locate the 59-end of a transcript by hybridizing an oligonucleotide primer to the RNA of interest, extending the primer with reverse transcriptase to the 5’-end of the transcript, and electrophoresing the reverse transcript to determine its size