Retrograde transport, is in the opposite direction (from the nerve ending to the cell body), occurs along microtubules at about 200 mm/day.

TLS Online TPP Program

#Id: 2837

#Unit 13. Methods in Biology

Maltose-Binding Protein (MBP) One-step purification can be achieved using amylose resin

TLS Online TPP Program

#Id: 2838

#Unit 13. Methods in Biology

Poly-Histidine Tag (His-tag) is also used as tag to separate protein

TLS Online TPP Program

#Id: 2839

#Unit 13. Methods in Biology

These histidine's are separated by using Nickel-chelate-nitrilotriacetate (Ni-NTA) chromatography column.

TLS Online TPP Program

#Id: 2840

#Unit 13. Methods in Biology

These are His-tag subsequently removed by cleavage with a reagent such as cyanogen bromide and the protein of interest purified by protein biochemical methods such as chromatography and polyacrylamide gel electrophoresis.

TLS Online TPP Program

#Id: 2841

#Unit 13. Methods in Biology

Phosphorylated protein or amino acid are separated by using immobilized metal affinity chromatography ( IMAC), or immuno-affinity using antibodies against phosphorylated amino acids or peptides

TLS Online TPP Program

#Id: 2842

#Unit 13. Methods in Biology

GLUT separation is also done with the help of affinity chromatography, normally glut protein are cloned into the vector and then the help of affinity chromatography