Nurturing Life Sciences
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#Unit 3. Fundamental Processes
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The replicase activity of DNA polymerase III was originally discovered by a conditional lethal mutation in the dnaE locus, which codes for the 130 kD, a subunit that possesses the DNA synthetic activity.
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The 3’–5’ exonucleolytic proofreading activity is found in E, coded by the dnaQ gene
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Mutations in dnaQ showed increased mutation frequency with by103-folds
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Proofreading typically decreases the error rate in replication from10-5 to 10-7 per base pair replicated.
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Repair Systems such as MMR reduces error rate of replication to 10-9 per base pair replicated