Use of biological organisms, such as, bacteria, fungi (usually), and plants to reduce or eliminate toxic pollutants from contaminated sites by degradation or assimilation in the atmosphere is called bioremediation. 

TLS Online TPP Program

#Id: 2624

#Unit 13. Methods in Biology

Type III restriction endonucleases are intermediate between the type I and type II enzymes; they cleave DNA in the immediate vicinity of their recognition sites.

TLS Online TPP Program

#Id: 2625

#Unit 13. Methods in Biology

Traditional method of labeling DNA is by the process of nick translation . 

TLS Online TPP Program

#Id: 2626

#Unit 13. Methods in Biology

Steps of nick translation- 

Low concentrations of DNase I are used to make occasional single-strand nicks in the double-stranded DNA that is intended as the gene probe.

DNA polymerase then fills in the nicks, using an appropriate dNTP, at the same time making a new nick to the 3′ side of the previous one.

TLS Online TPP Program

#Id: 2627

#Unit 13. Methods in Biology

Types and examples of typical enzymes used in the manipulation of nucleic acids

TLS Online TPP Program

#Id: 2628

#Unit 13. Methods in Biology

Types and examples of typical enzymes used in the manipulation of nucleic acids

TLS Online TPP Program

#Id: 2629

#Unit 13. Methods in Biology

End-labelling of a gene probe at the 5′ end with alkaline phosphatase and polynucleotide kinase.