The total mass of the pore structure is 60,000– 80,000 kDa in vertebrates, which is about 16 times larger than a ribosome.

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#Id: 2789

#Unit 13. Methods in Biology

The second component is the time the stationary phase retains the analyte, referred to as the adjusted retention time, t’R.

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#Id: 2790

#Unit 13. Methods in Biology

The ratio VS/VM is referred to as the volumetric phase ratio, b. Hence:

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#Id: 2791

#Unit 13. Methods in Biology

notSelectivity or separation factor, a, which can also be viewed as simply the relative retention ratio for the two analytes:

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#Id: 2792

#Unit 13. Methods in Biology

Chromatography columns are considered to consist of a number of adjacent zones in each of which there is sufficient space for an analyte to completely equilibrate between the two phases, Each zone is called a theoretical plate (having no N).

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#Id: 2793

#Unit 13. Methods in Biology

The length of column containing one theoretical plate is referred to as the plate height, H, which has units of length normally in micrometers.

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#Id: 2794

#Unit 13. Methods in Biology

Plate height is simply related to the width of the analyte peak, expressed in terms of its standard deviation s , and the distance it travelled within the column, x. Specifically