The ER captures selected proteins from the cytosol as they are being synthesized. These are of two types:

Transmembrane proteins

Water-soluble proteins

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#Id: 868

#Unit 13. Methods in Biology

PCR amplification is mathematically counted as 2n So 10 cycle leads to 1024 times amplification

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#Id: 869

#Unit 13. Methods in Biology

The efficiency of PCR can be calculated by using Y = X (1+ E)n X is number of templates, E is mean PCR efficiency, n is number is PCR cycle. 

 

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#Unit 13. Methods in Biology

Variants of PCR has been devised for 

a. Efficiency

b. Sequence specific amplification

c. Mutation analysis

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#Unit 13. Methods in Biology

Variants of PCR has been devised to enhance efficiency are 

a. qPCR with SYBR, Taqman and MB 

b. Nested PCR

c. Touchdown PCR

d. Hot-start PCR 

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#Unit 13. Methods in Biology

Variants of PCR has been devised for sequence specific amplification are Inverse PCR, Anchored PCR, RT-PCR RACE (3’ and 5’) RLM-RACE, Multiplex PCR, Colony PCR.

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#Unit 13. Methods in Biology

Variants of PCR has been devised for mutational analysis AS-PCR, Site-directed-mutagenesis (Oligonucleotide-directed Mutagenesis), overlap extension PCR, Megaprimer PCR.