The amino group at C-2 bears a fatty acid in amide linkage.

The fatty acid is usually saturated or monounsaturated, with 16, 18, 22, or 24 carbon atoms.

Ceramide is the parent compound for this group.

TLS Online TPP Program

#Id: 2746

#Unit 13. Methods in Biology

A comparison between RFLP and RAPD markers for genome mapping in plan

TLS Online TPP Program

#Id: 2747

#Unit 13. Methods in Biology

RAPDs (Random amplified polymorphic DNAs) are obtained by using a PCR (polymerase chain reaction) equipment or a thermal cycler. 

TLS Online TPP Program

#Id: 2748

#Unit 13. Methods in Biology

The RAPD technique is based on PCR, which uses a DNA polymerase that is resistant to the high temperature needed for DNA denaturation; this permits the enzyme to be used repeatedly during the amplification process. 

TLS Online TPP Program

#Id: 2749

#Unit 13. Methods in Biology

RAPDs are more sensitive than RFLPs to experimental conditions making them more difficult to be consistent and reproducible. 

TLS Online TPP Program

#Id: 2750

#Unit 13. Methods in Biology

Amplified Fragment length Polymorphism (AFLP) shares some features of both RFLP and RAPD analyses.

TLS Online TPP Program

#Id: 2751

#Unit 13. Methods in Biology

AFLP uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.