Most of the lysosome membrane proteins, are highly glycosylated, which helps to protect them from the lysosome proteases

#Id: 2469

#Unit 13. Methods in Biology

Phenomenon of Electroendosmosis is largely applicable in Free Solution & Capillary.

#Id: 2470

#Unit 13. Methods in Biology

Electrophoretic mobility and Electroendosmosis force combinedly functions in Capillary zone Electrophoresis

#Id: 2471

#Unit 13. Methods in Biology

In electrophoretic separation the phenomenon of electroendosmosis (also known as electro-osmotic flow) which is due to the presence of charged groups on the surface of the support medium.

#Id: 2472

#Unit 13. Methods in Biology

Three basically different electrophoretic separation methods are performed in practice nowadays

 Electrophoresis, sometimes called Zone Electrophoresis (ZE) GE

 Isotachophoresis (ITP) SDS PAGE

Isoelectric focusing (IEF).

#Id: 2473

#Unit 13. Methods in Biology

In ZE a homogeneous buffer system is used over the whole separation time and range so as to ensure a constant pH value.

#Id: 2474

#Unit 13. Methods in Biology

In ITP, the separation is carried out in a discontinuous buffer system. The ionized sample migrates between a leading electrolyte with a high mobility and a terminating – sometimes called trailing – ion with a low mobility, all of them migrating with the same speed.