Nurturing Life Sciences
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#Unit 13. Methods in Biology
(CFSE) or carboxyfluorescein diacetate succinimidyl ester (CFDASE) is nontoxic to cells hence retained till many cycles
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The 51Cr Release Assay is used to measure Cell death not apoptosis
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Normal cells treated with sodium chromate allowed to absorb and induced to death signals as T-CTLs Death of the target cells is indicated by the release of 51Cr into the supernatant
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FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS
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The TUNEL Assay measures Apoptotically Generated DNA Fragmentation.