Phosphorylated Knl1 binds the checkpoint protein kinase Bub1-Bub3 and checkpoint protein BubR1. These three proteins, recruit the Mad1-Mad2 complex to the kinetochore.

TLS Online TPP Program

#Id: 6686

#Unit 3. Fundamental Processes

Alternative splicing is often associated with weak splice sites.

TLS Online TPP Program

#Id: 6687

#Unit 3. Fundamental Processes

Binding of SR proteins to ESEs promotes or stabilizes U1 binding to the 5’ splice site and U2AF binding to the 3’ splice site.

TLS Online TPP Program

#Id: 6688

#Unit 3. Fundamental Processes

The Nova and Fox families of RNA-binding proteins can promote or suppress splice site selection in a context-dependent fashion.

TLS Online TPP Program

#Id: 6689

#Unit 3. Fundamental Processes

Trans-splicing is rare in multicellular eukaryotes, it occurs as the primary mechanism to process precursor RNA into mature, translatable mRNAs in some organisms, such as trypanosomes and nematodes.

TLS Online TPP Program

#Id: 6690

#Unit 3. Fundamental Processes

 The SL RNA provides an exon that is connected to the first exon of an mRNA by trans-splicing. The reaction involves the same interactions as nuclear cis splicing but generates a Y-shaped RNA instead of a lariat.

TLS Online TPP Program

#Id: 6691

#Unit 3. Fundamental Processes

In C. elegans, about 70% of genes are processed by the trans-splicing mechanism