#Id: 4630
#Unit 13. Methods in Biology
Antigen can be detected or measured by a sandwich ELISA .In this technique, the antibody (rather than the antigen) is immobilized on a microtiter well.
A sample containing antigen is added and allowed to react with the immobilized antibody. After the well is washed, a second enzyme- linked antibody specific for a different epitope on the antigen is added and allowed to react with the bound antigen
After any free second antibody is removed by washing, substrate is added, and the colored reaction product is measured.
