Practice Questions

TLS Online TPP Program

#Question id: 16137

You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.

By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:

How would you use the sequence information presented in to design two new STSs (with new PCR primer pairs) to replace STS5? (Call the new ones STS51 and STS52. STS51 should be present (+) in BAC B, and STS52 should be present (+) in BAC F.)

#Unit 13. Methods in Biology

Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS52 not STS51.
Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify both simultaneously STS51 and STS52.
Primers that include the homologous sequence in STS51 and STS52. These primers should specifically amplify only STS51 not STS52.
Primers that include the non-homologous sequence in STS51 and STS52. These primers should specifically amplify either STS51 or STS52.

More Questions

TLS Online TPP Program

#Question id: 23883

#Unit 13. Methods in Biology

A paleontologist has recovered a bit of tissue from the 400-year-old preserved skin of an extinct dodo (a bird). The researcher would like to compare a specific region of the DNA from the sample with DNA from living birds. Which of the following would be most useful for increasing the amount of dodo DNA available for testing?

TLS Online TPP Program

#Question id: 5529

#Unit 5. Developmental Biology

In humans, organogenesis in the embryo is basically accomplished by the end of which period of the pregnancy?

TLS Online TPP Program

#Question id: 12592

#Unit 10. Ecological Principles

Use the following diagram of five islands formed at around the same time near a particular mainland and MacArthur and Wilson's island biogeography principles to answer the following questions.

Which island would likely have the greatest species diversity?

TLS Online TPP Program

#Question id: 19741

#Unit 3. Fundamental Processes

Telomerase protein component with catalytic activity is encoded by

TLS Online TPP Program

#Question id: 26559

#Unit 3. Fundamental Processes

Which of the following is correct regarding piRNAs

a. piRNAs which are 24–34 nucleotides long

b. It does not involve a dsRNA precursor

c. It involve a dsRNA precursor