In Gradient Gel system gel from 5 to 25 % is used, which created sieving effect due to this effect

TLS Online TPP Program

#Question id: 26513

#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using

TLS Online TPP Program

#Question id: 26514

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits which are

TLS Online TPP Program

#Question id: 26515

#Unit 3. Fundamental Processes

Which of the two technique can be used to separate Eukaryotic RNA pol     

a. DEAE Column 

b. epitope tagging

c. Density gradient centrifugation 

d. UV-vis

TLS Online TPP Program

#Question id: 26516

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG and myc, so FLAG tagged protein  can be separated by using

TLS Online TPP Program

#Question id: 26517

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell. Alexa- 488-labeled antibody –emit……., Alexa-568-labeled antibody emits ……….respectivel

TLS Online TPP Program

#Question id: 26518

#Unit 3. Fundamental Processes

A significant difference between the transcription of eukaryotic and prokaryotic RNAs is, choose the correct option

a.In bacteria, transcription takes place on a DNA template

b.In bacteria takes place on a chromatin template as a domain 

c.In eukaryotes, transcription takes place on a chromatin template as a domain 

d.In eukaryotes transcription takes place on a DNA template