Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle. After passing through flow cytometer a graph is observed

  

In this graph A, B, C, and D represent a cell stage which are

TLS Online TPP Program

#Question id: 23146

#Unit 13. Methods in Biology

A phenomenon of absorption of light at a certain wavelength and then emission at longer wavelength is known as

TLS Online TPP Program

#Question id: 23147

#Unit 13. Methods in Biology

In fluorescence microscopy why the fluorescence occurs at longer wavelength when there shorter   wavelength of light is applied?

TLS Online TPP Program

#Question id: 23148

#Unit 13. Methods in Biology

There are two proteins A and B to be visualized under fluorescence microscopy. Both of them are excited with same wavelength of light but A emits longer wavelength. Hence we can conclude that 

1. A is denser molecule than B

2. B is denser molecule than A

3. A is lighter molecule than B

4. B is lighter molecule than A

Which of the following option is incorrect?

TLS Online TPP Program

#Question id: 23149

#Unit 13. Methods in Biology

In fluorescence microscopy a usually used fluorochrome that is fura-2 is sensitive to which ions?

TLS Online TPP Program

#Question id: 23150

#Unit 13. Methods in Biology

How fura-2 dye moves inside the cell through plasma membrane?

TLS Online TPP Program

#Question id: 23151

#Unit 13. Methods in Biology

If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?

1. Fluorescence will decrease

2.  Fluorescence will increase

3. Emission of light at higher wavelength

4. Emission of light at lower wavelength

Which of the following options are correct?