TLS Online TPP Program

#Question id: 18846


For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

#Unit 14. Methods in Biology
  1. Affinity chromatography (AC) followed by size exclusion chromatography (SEC)
  2. AC only
  3. AC followed by ion-exchange (IEX) followed by SEC
  4. AC followed by IEX, followed by hydrophobic interaction (HIC) and then SEC
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TLS Online TPP Program

#Question id: 10253

#Unit 6. System Physiology – Plant

Two pathways for the splitting of sucrose are known in plants, both of which take part in the use of sucrose from phloem unloading;

TLS Online TPP Program

#Question id: 10254

#Unit 6. System Physiology – Plant

Sucrose is split into its two monosaccharide units—glucose and fructose—which can readily enter the glycolytic pathway. Two pathways for the splitting of sucrose are known in plants, both of which take part in the use of sucrose from phloem unloading; Invertase pathway and sucrose synthase pathway, some statements are given about these pathways, which of the following is incorrect?

TLS Online TPP Program

#Question id: 10255

#Unit 6. System Physiology – Plant

In sucrose synthase combines sucrose with UDP to produce fructose and UDP-glucose in the cytosol. What is the function of UDP-glucose pyrophosphorylase?

TLS Online TPP Program

#Question id: 10256

#Unit 6. System Physiology – Plant

Both sucrose synthase and invertases can degrade sucrose for glycolysis, what will happen if one of the enzymes is absent or mutant?

TLS Online TPP Program

#Question id: 10273

#Unit 6. System Physiology – Plant

The existence of different pathways that serve a similar function and can replace each other without a clear loss in function is called,

TLS Online TPP Program

#Question id: 10274

#Unit 6. System Physiology – Plant

In plastids, a partial glycolysis occurs that produces metabolites for plastidial biosynthetic reactions, which of the following is not synthesized in the plastid?