You are conducting genetic linkage studies of an autosomal recessive disease whose chromosomal location has not been firmly established. You are focused on two SSR markers that may be linked to each other and to the disease. Here are two families in which some individuals are affected:

                             

Calculate LOD scores for linkage at θ= 0.02 between the disease and SSR42 in Family 2.

TLS Online TPP Program

#Question id: 26514

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits which are

TLS Online TPP Program

#Question id: 26515

#Unit 3. Fundamental Processes

Which of the two technique can be used to separate Eukaryotic RNA pol     

a. DEAE Column 

b. epitope tagging

c. Density gradient centrifugation 

d. UV-vis

TLS Online TPP Program

#Question id: 26516

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG and myc, so FLAG tagged protein  can be separated by using

TLS Online TPP Program

#Question id: 26517

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell. Alexa- 488-labeled antibody –emit……., Alexa-568-labeled antibody emits ……….respectivel

TLS Online TPP Program

#Question id: 26518

#Unit 3. Fundamental Processes

A significant difference between the transcription of eukaryotic and prokaryotic RNAs is, choose the correct option

a.In bacteria, transcription takes place on a DNA template

b.In bacteria takes place on a chromatin template as a domain 

c.In eukaryotes, transcription takes place on a chromatin template as a domain 

d.In eukaryotes transcription takes place on a DNA template

TLS Online TPP Program

#Question id: 26519

#Unit 3. Fundamental Processes

A regulatory site that binds more negative regulators than positive regulators to control transcription is called a