chaotropic agents such as guanidinium ion and urea, the efficiency of the chaotropic agent is measured in terms of-

TLS Online TPP Program

#Question id: 21411

#Unit 12. Biochemical Engineering

Increasing the agitation in a reactor increases oxygen transfer primarily because

TLS Online TPP Program

#Question id: 21412

#Unit 12. Biochemical Engineering

In a continuous culture of Saccharomyces cerevisiae, the cell density is 30 gL1 (DCW), the dilution rate (D) is 0.4 h^-1 and substrate uptake rate (q) is 18 gL^-1 h^-1. The cell yield coefficient Yx/s will be:

TLS Online TPP Program

#Question id: 21413

#Unit 12. Biochemical Engineering

Aqueous two phase partitioning (ATPS) is used for the recovery of an enzyme from the cell free culture filtrate on addition of PEG2000 and dextran. The mixture separates into two phases with a partition coefficient for the enzyme = 4.2. The maximum possible enzyme recovery, when the volume ratio of the upper to lower phases is 5.0 will be:

TLS Online TPP Program

#Question id: 21414

#Unit 12. Biochemical Engineering

A fermentation medium is being cooled from 70⁰ to 32⁰ in a double pipe heat exchanger. Cooling fluid flowing counter currently with this stream is heated from 20⁰ to 46⁰ . The log mean temperature difference (in⁰ ) for the two streams is:

TLS Online TPP Program

#Question id: 21619

#Unit 12. Biochemical Engineering

For a microbial culture, if the doubling time is 0.231 h, the specific growth rate (in h-1) will be (assume that the endogenous metabolism is negligible)

TLS Online TPP Program

#Question id: 21634

#Unit 12. Biochemical Engineering

The equation for aerobic production of acetic acid from ethanol is:

CH2OH+O2→CH3CO2H+H2O.

(ethanol) (acetic acid)

Acetobacter aceti bacteria are added to vigorously-aerated medium containing 10 g.14 ethanol. After some time, the ethanol concentration is 2 g.-2 and 7.5 g.11 of acetic acid is produced. What is the observed yield (Yoing-) and theoretical yield (Yting-8") of acetic acid from ethanol?