HOTAIR is a lncRNA whose gene is found in the HoxC cluster in humans, but it acts by regulating expression of the HoxD genes on another chromosome (in trans) by recruiting to that locus Polycomb Repressive Complex 2 (PCR2). Which of the following processes is incorrect regarding this regulation?

TLS Online TPP Program

#Question id: 34179

#Unit 3. Fundamental Processes

A. At the replication fork, the dsDNA in front of the fork becomes increasingly positively supercoiled.

B. At the replication fork, the dsDNA in front of the fork becomes increasingly negatively supercoiled.

C. The “swivelase” that prevents the accumulation of positive supercoils ahead of the replication fork.

D. The “swivelase” that prevents the accumulation of negative supercoils ahead of the replication fork.

Which of the following combination of statements is correct

TLS Online TPP Program

#Question id: 34180

#Unit 3. Fundamental Processes

Many proteins that act at the replication fork interact tightly. Which of the following interactions participate in DNA protein interactions

A. Sequence dependent interaction

B. Negative charge and structure of the phosphate backbone

C. Hydrogen-bonding residues in the minor groove for the palm domain

D. Hydrophobic stacking interactions between the bases for SSBs

Choose true and false

TLS Online TPP Program

#Question id: 34181

#Unit 3. Fundamental Processes

A. In mammalian systems the DNA polymerase does not have a 5’–3’ exonuclease activity

B. In eukaryotes Synthesis of an Okazaki fragment displaces the RNA primer of the preceding fragment in the form of a “flap.”

C. FEN1 functions as an endonuclease and also has a 5’–3’ exonuclease activity

D. FEN1 prevents deletions or duplications in the genome.

Choose true and false

TLS Online TPP Program

#Question id: 34182

#Unit 3. Fundamental Processes

A. The E. coli DNA polymerase III catalytic core contains three subunits, including a catalytic subunit and a proofreading subunit.

B. The DNA Pol III holoenzyme has at least two catalytic cores, a processivity clamp, and a dimerization clamp-loader complex.

C. A clamp loader places the processivity subunits on DNA, where they form a circular clamp around the nucleic acid.

D. Two catalytic core is associated with each template strand.

Which of the following combination is correct

TLS Online TPP Program

#Question id: 34183

#Unit 3. Fundamental Processes

A. A & B can be leading strands

B. B & C can be lagging strands

C. Ligase mutants will affect ether A & D or B & C

D. E and F are origins

E. G is replication fork

Choose true and false

TLS Online TPP Program

#Question id: 34184

#Unit 3. Fundamental Processes

Which of the following is incorrect option