In a cDNA library the probability of finding the desired clone depends on ________.

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#Question id: 12181

#Unit 2. Cellular Organization

Following statements are regarding to the CFTR.

A. cystic fibrosis transmembrane conductance regulator allows Cl− ions to flow out of the cell, following their concentration gradient.

B. ATP binding to CFTR’s two nucleotide-binding domains appears to close the Cl− channel, and the hydrolysis of one ATP opens it (the other ATP remains intact).

C. the CFTR channel can open only if its regulatory domain (which is unique among ABC transporters) has been phosphorylated, thus regulating the flow of ions across the membrane.

D. The maintenance of electrical neutrality requires that the Cl− ions transported by the CFTR be accompanied by positively charged ions, mainly Na+. The transported ions are osmotically accompanied by water, thus maintaining the proper level of fluidity in secretions of the airways, intestinal tract, and the ducts of the pancreas, testes, and sweat glands.

Which of the following combination is correct?

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#Question id: 12182

#Unit 2. Cellular Organization

The cell cycle is controlled by protein kinases that comprise a catalytic subunit and a regulatory subunit. Which of the following statement is correct regarding to these subunits?

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#Question id: 12183

#Unit 2. Cellular Organization

Following statements are regarding to regulation of S phase and mitotic cyclin levels in budding yeast.

A. In late anaphase, the anaphase-promoting complex (APC/C) ubiquitinylates S phase and mitotic cyclins.

B. In late anaphase, the anaphase-promoting complex (APC/C) phosphorylates S phase and mitotic cyclins.

C. During exit from mitosis and G1, Cdh1 is dephosphorylated and active; during S phase and mitosis, Cdh1 is phosphorylated and dissociates from APC/C, and APC/C becomes inactive.

D. During exit from mitosis and G1, Cdh1 is phosphorylated and active; during S phase and mitosis, Cdh1 is dephosphorylated and dissociates from APC/C, and APC/C becomes inactive.

E. The G1/S phase CDKs, which themselves are not APC/CCdh1 substrates, phosphorylate Cdh1 at the G1–S phase transition.

F. The G1/S phase CDKs, which themselves are not APC/CCdh1 substrates, ubiquitinylates Cdh1 at the G1–S phase transition.

Which of the following combination is correct?

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#Question id: 12184

#Unit 2. Cellular Organization

Following graph represent Control of S phase onset in S. cerevisiae by regulated proteolysis of the S phase inhibitor Sic1. 

Which of the following is correct according to graph?

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#Question id: 12185

#Unit 2. Cellular Organization

Mitotic CDKs affect nuclear envelope components by specific mechanism. Which of the following statement is not related to this mechanism?

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#Question id: 12186

#Unit 2. Cellular Organization

In 2001, the Nobel Prize in Physiology or Medicine was awarded to three cell cycle scientists. Paul Nurse was recognized for his studies with the fission yeast S. pombe, in particular for the discovery and characterization of the wee1+ gene. What did the characterization of the wee1+ gene tell us about cell cycle control?