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TLS Online TPP Program

#Question id: 23682

Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

#Unit 13. Methods in Biology
  1. insert the fragments cut with X directly into the plasmid without cutting the plasmid.
  2. cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid.
  3. cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
  4. cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.
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TLS Online TPP Program

#Question id: 3343

#Unit 11. Evolution and Behavior

Following genotype frequency as hardy Weinberg genetic equilibrium in figure. What is the frequency of A1 and A2 allele?
TLS Online TPP Program

#Question id: 3521

#Unit 8. Inheritance Biology

Which of the following is incorrect about independent assortment?
TLS Online TPP Program

#Question id: 3534

#Unit 8. Inheritance Biology

Start with a collection of mutants obtained using selections or screens and then characterize their wild functions at the molecular level called ----A------, alternatively, start with a set of specific DNA sequences, mutate them, and observe the phenotypes called --B-----.
TLS Online TPP Program

#Question id: 19369

#Unit 12. Applied Biology

Genes are transferred from prokaryotes into plants this phase is considered as 
TLS Online TPP Program

#Question id: 19044

#Unit 13. Methods in Biology

Which of the following techniques would be most useful to identify as well as quantify the presence of a known impurity in a drug substance?