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TLS Online TPP Program

#Question id: 23682

Assume that you are trying to insert a gene into a plasmid. Someone gives you a preparation of genomic DNA that has been cut with restriction enzyme X. The gene you wish to insert has sites on both ends for cutting by restriction enzyme Y. You have a plasmid with a single site for Y, but not for X. Your strategy should be to

#Unit 13. Methods in Biology
  1. insert the fragments cut with X directly into the plasmid without cutting the plasmid.
  2. cut the plasmid with restriction enzyme X and insert the fragments cut with Y into the plasmid.
  3. cut the DNA again with restriction enzyme Y and insert these fragments into the plasmid cut with the same enzyme.
  4. cut the plasmid twice with restriction enzyme Y and ligate the two fragments onto the ends of the DNA fragments cut with restriction enzyme X.
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TLS Online TPP Program

#Question id: 10302

#Unit 6. System Physiology – Plant

During respiration, given below some of the reaction of glycolysis are irreversible which are bypass during gluconeogenesis,which of the following ics correcty involve in
TLS Online TPP Program

#Question id: 10303

#Unit 6. System Physiology – Plant

During glycolysis, if radiolabelled the glucose at 3 or 4 carbon then what will be the correct prediction about radiolabelling in the cycle?
TLS Online TPP Program

#Question id: 10304

#Unit 6. System Physiology – Plant

Pyruvate is coverted into the Acetyl-CoA, Acetyl-CoA contain the acetyl group which are comes from the pyruvate and Coenzyme A (CoA) that contain other groups such as;

a) A reactive thiol (—SH) group that is critical to the role of CoA as an acyl carrier in a number of metabolic reactions

b) contain pantothenic acid,  A hydroxyl group of pantothenic acid is joined to a modified ADP moiety by a phosphate ester bond

c) pantothenic acid carboxyl group is attached to β-mercaptoethylamine in amide linkage

d) Hydroxyl group at the 3’ position of the ADP moiety has a phosphoryl group not present in free ADP

which of the following correct observation about Coenzyme A (CoA)?
TLS Online TPP Program

#Question id: 10305

#Unit 6. System Physiology – Plant

Pyruvate dehydrogenase complex Consists of three Distinct enzymes and it requires Coenzymes, these are;
TLS Online TPP Program

#Question id: 10306

#Unit 1. Molecules and their Interaction Relevant to Biology

pyruvate dehydrogenase (PDH) complex requires five different coenzymes or prosthetic groups and these enzymesalso require four different vitamins in human nutrition are vital components of this system:
TLS Online TPP Program

#Question id: 10306

#Unit 6. System Physiology – Plant

pyruvate dehydrogenase (PDH) complex requires five different coenzymes or prosthetic groups and these enzymesalso require four different vitamins in human nutrition are vital components of this system: