A consensus sequence

TLS Online TPP Program

#Question id: 13075

#Unit 13. Methods in Biology

Radioisotopes must be used in RFLP and RAPD markers

TLS Online TPP Program

#Question id: 13076

#Unit 13. Methods in Biology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.

TLS Online TPP Program

#Question id: 13077

#Unit 13. Methods in Biology

The DNA probes for RFLP may NOT be obtained from

TLS Online TPP Program

#Question id: 13078

#Unit 13. Methods in Biology

RFLPs have several unique advantages except this one;

TLS Online TPP Program

#Question id: 13079

#Unit 13. Methods in Biology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,

TLS Online TPP Program

#Question id: 13080

#Unit 13. Methods in Biology

In RAPDs amplification will take place only of those regions of the genome that have the