In humans, PKU (phenylketonuria) is a recessive disease caused by enzyme inefficiency at step A in the following simplified reaction sequence, and AKU (alkaptonuria) is a recessive disease due to enzyme inefficiency in one of the steps summarized as step B here:A person with PKU and homozygous for normal of other character was married a person with AKU and homozygous for normal of other character. What is expected phenotypes in their children?

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#Question id: 18853

#Unit 13. Methods in Biology

Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

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#Question id: 18854

#Unit 13. Methods in Biology

What properties of a protein does hydrophobic interaction chromatography exploit for purification?

TLS Online TPP Program

#Question id: 18855

#Unit 13. Methods in Biology

to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

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#Question id: 18856

#Unit 13. Methods in Biology

A scientist constructs a recombinant bacterial plasmid that contains a gene conferring resistance to the antibiotic ampicillin (amp). The scientist transforms cultures of E. coli bacteria with the recombinant plasmid, then incubates the cultures overnight on plates containing media without ampicillin (amp-) and with ampicillin (amp+).

Assuming the transformation protocol was successful, which of the following best predicts the bacterial growth pattern expected after the incubation period?

 

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#Question id: 18857

#Unit 13. Methods in Biology

Embryonic lethality that sometimes occurs in knockout models can be avoided by...

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#Question id: 18858

#Unit 13. Methods in Biology

Where in the genome are loxP sites located in a Cre/Lox model?