Match the correct animal and their mechanism of germplasm specification.

Animal	Specification of germ cells
a. Drosophila	i. germplasm is characterized by polar granules and PIE-1 protein, and segregates into P4 during cleavage
b. Xenopus	ii. germplasm localized in vegetal region of egg
c. mammals	iii. germplasm specified by oskar at posterior end of egg
d. c. elegans	iv. Have no germplasm

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#Question id: 12921

#Unit 13. Methods in Biology

Match the following:-

A. Gene probe production.              i. Novel proteins

B. Genome mapping.                       ii. PCR mutagenesis

C. Protein Engineering.                 iii. Sequence tagged sites

D. Gene Editing.                                iv. Use with blots

TLS Online TPP Program

#Question id: 12922

#Unit 13. Methods in Biology

 Match the following:-

1. Ligase chain reaction.              i. Mutation detection

2. Nucleic acid sequence based   amplification.                            ii.Non isothermal                   

3. Loop mediated isothermal amplification.                      iii. Viral detection

4. Branched DNA amplification.   iv.parasitic disease

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#Question id: 12923

#Unit 13. Methods in Biology

Name the technology which is used as a means of identifying the initial concentrations of DNA or cDNA template used.

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#Question id: 12924

#Unit 13. Methods in Biology

In order to quantify unknown DNA templates, a standard dilution is prepared using DNA of known concentration. As the DNA accumulates during the early exponential phase of the reaction,an arbitrary point is taken where each of the diluted DNA samples cross. This is termed as_____

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#Question id: 12925

#Unit 13. Methods in Biology

Another name of TaqMan assay is ____

TLS Online TPP Program

#Question id: 12926

#Unit 13. Methods in Biology

PCR is undertaken with a fluorescence reporter / quencher pair called______