Enzymes their location and cofactor along with the inhibitors is given 

a. Citrate synthase Located in Matrix space uses CoA as cofactor and can be inhibited by Monofluoroacetyl-CoA

b. Aconitase Located in Inner membrane uses Fe2+ as cofactor and can be inhibited by Fluoro acétate

c. Isocitrate dehydrogenase Located Matrix space in uses NAD, NADP Mg2+. Mn2+ as cofactor and can be inhibited by ATP

d. Succinate dehydrogenase Located in Inner membrane uses FAD as cofactor and can be inhibited by Malonate, Oxaloacetate

Which of the following is correct

TLS Online TPP Program

#Question id: 19885

#Unit 13. Methods in Biology

The plasmid used by Cohen and Boyer fr their transformation experiment was

TLS Online TPP Program

#Question id: 19886

#Unit 13. Methods in Biology

Gene cloning refers to the

I) Production of large number of copies of the gene being cloned

II) Production of asexual progeny from a single individual or a cell 

III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

TLS Online TPP Program

#Question id: 19887

#Unit 13. Methods in Biology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Unit 13. Methods in Biology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19889

#Unit 12. Applied Biology

The genetic monitoring and disease diagnosis are examples for ______________________ sensor?

TLS Online TPP Program

#Question id: 19890

#Unit 12. Applied Biology

QCM is a kind of a piezoelectric biosensor which detects mass change. What is the standard form of QCM biosensor ?