TLS Online TPP Program
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TLS Online TPP Program
#Question id: 12826
#General Aptitude
ABC is a triangle with base AB. D is a point on AB such that AB= 5 and DB = 3. What is the ratio of the area of DADC to the area of DABC ?
TLS Online TPP Program
#Question id: 13091
#Unit 13. Methods in Biology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
Which colony’s plasmid do you actually want to use for your studies?
TLS Online TPP Program
#Question id: 11994
#Unit 10. Ecological Principles
In mountainous areas of western North America, north-facing slopes would be expected to
TLS Online TPP Program
#Question id: 24674
#Unit 2. Cellular Organization
Membrane lipids may
a. spontaneously flip-flop from one membrane leaflet to the other.
b. move laterally within one membrane leaflet.
c. aggregate with membrane proteins to form lipid rafts.
d. be unequally distributed in the two membrane leaflets.
e. be present in varying amounts in different cellular membranes
TLS Online TPP Program
#Question id: 1038
#Unit 4. Cell Communication and Cell Signaling
Lister developed his ideas on prevention of infection during medical procedures after studying the work of