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TLS Online TPP Program
#Question id: 7575
#General Aptitude
What is the distance between Alankar’s house and office, if he will reach office late by 20 minutes, traveling at 10 km/hr and will reach early by 15 minutes, traveling at 15 km/hr?
TLS Online TPP Program
#Question id: 12991
#Unit 1. Molecules and their Interaction Relevant to Biology
Choose the correct statements
a. Secondary structures are stabilized by hydrogen bonds.
b. Hydrogen bond always formed between non bonded atoms.
c. the folded length of an amino acid in α-helix reduced almost 3 times than linear structure.
d. Only dihedral angle changes during the formation of secondary structure while bond length remains unchanged
TLS Online TPP Program
#Question id: 13093
#Unit 13. Methods in Biology
You are studying a specific gene in yeast, and you want to express that yeast gene in E. coli. Your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You transform your ligation planned in which two restriction enzymes would you use to design a way to get the insert into the vector if you had to use two enzymes simultaneously, into bacteria and plate the bacteria on Petri plates containing ampicillin. (You actually transform six different ligation mixtures, which are described below, into six different populations of cells, and plate each transformation onto a different plate, because you want to do all of the correct controls.) The next day you come in to lab to look at how many colonies of bacteria are on each plate. You are really excited, because the number of colonies you see on each plate tells you that the entire procedure worked! Which of the three following patterns of number of colonies did you see in order to conclude that you had a successful transformation?
In this table, DV = digested vector. DYG = digested yeast genome.
TLS Online TPP Program
#Question id: 12468
#Unit 10. Ecological Principles
If the sex ratio in a population is significantly different from 50:50, then which of the following will always be true?
TLS Online TPP Program
#Question id: 18699
#Unit 13. Methods in Biology
BamHI recognizes 6 base pairs of DNA in a palindromic DNA sequence. If the first part of the sequence is given below, what is the rest of it?