#Question id: 4265
#Unit 3. Fundamental Processes
48S pre initiation complex includes
#Question id: 27635
#Unit 1. Molecules and their Interaction Relevant to Biology
#Question id: 15681
#Unit 2. Cellular Organization
#Question id: 2909
#Unit 2. Cellular Organization
The DNA can be labeled and used to probe a microarray, yielding a map of genomic sequences to which those particular nucleosomes bind. Because microarrays are often referred to as chips, this technique is called a ChIP-chip experiment. This experiment is designed to reveal the genomic DNA sequences to which a particular histone variant binds.
I. Chromatin is isolated from the cells and digested briefly with micrococcal nuclease (MNase).
II. A histone variant with an epitope tag is introduced into a particular cell type, where it is incorporated into nucleosomes.
III. The DNA bound by nucleosomes is protected from digestion, but the linker DNA is cleaved, releasing segments of DNA bound to one or two nucleosomes.
IV. The pattern of hybridization on the microarray reveals the DNA sequences bound by the nucleosomes with the histone variant.
V. Antibody is added, and the nucleosomes containing the epitope-tagged histone variant are selectively precipitated.
VI. The DNA in these nucleosomes is extracted from the precipitate, labeled, and used to probe a microarray representing all or part of the genomic sequences of that particular cell type.
Arrange the following statements in the correct order in which they are done.
#Question id: 4798
#Unit 8. Inheritance Biology
In goats, a beard is produced by an autosomal allele that is dominant in males and recessive in females. We’ll use the symbol Bb for the beard allele and B for the beardless allele. Another independently assorting autosomal allele that produces a black coat (W) is dominant over the allele for white coat (w). A cross allowed between BBbWw (male) X BBbWw (female). What is proportional of female progeny will beard with black coat?