Following statements are regarding to the mechanism of migration of pollen tube.

A. The embryo sac is the product of three mitotic divisions of the diploid megaspore; it comprises seven cells and eight haploid nuclei.

B. The two polar nuclei in the central cell will fuse with the second sperm nucleus and produce the endosperm that will nourish the egg. The other six cells, including the egg, contain one haploid nucleus each.

C. After compatible pollen germinates, the pollen tube grows toward the micropyle.

D. The three cells from the pollen are connected to one another at the tip of the tube, and waves of sodium ions play a key role in the growth of the tube.

Which of the following combinations are correct?

TLS Online TPP Program

#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?

TLS Online TPP Program

#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?

TLS Online TPP Program

#Question id: 23165

#Unit 13. Methods in Biology

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?

TLS Online TPP Program

#Question id: 23167

#Unit 13. Methods in Biology

A secondary antibody can be generated by immunizing a goat with antibody which segment that is common to all rabbit IgG antibodies called goat anti-rabbit.

TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?