Practice Questions

TLS Online TPP Program

#Question id: 31376

In S. cerevisiae, DNA replication is tightly controlled, and DNA should replicate once per cell cycle. Choose the INCORRECT statement regarding why the cells do not re-replicate their DNA in the S-phase?

#Unit 2. Cellular Organization

Because S phase CDKs phosphorylate several components of the MCM helicase-loading machinery.
Because phosphorylation leads to export of Cdt1 from the nucleus, and degradation of Cdc6 by the SCF ubiquitin-protein ligase in yeast.
Because Cdt1 which helps in the recruitment of MCM proteins in the Gl phase is inactivated by geminin in the S-phase of the cell cycle.
Because MCM helicases are loaded onto DNA to form pre-RCs only when they are phosphorylated.

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TLS Online TPP Program

#Question id: 13103

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

Why is it that some people show only one band?

TLS Online TPP Program

#Question id: 13104

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.

Why is it that some people show two bands?

TLS Online TPP Program

#Question id: 13105

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

Given the data so far, for which of the following statement is correct

TLS Online TPP Program

#Question id: 13106

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

Why is it that some people only have one band?

TLS Online TPP Program

#Question id: 13107

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

Why is it that some people show two bands?

TLS Online TPP Program

#Question id: 13108

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.)

You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

Why is it that some people show three bands?