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TLS Online TPP Program
#Question id: 28989
#Unit 7. System Physiology – Animal
Which of the following is the major mechanism to terminate the actions of norepinephrine?
TLS Online TPP Program
#Question id: 28696
#Unit 2. Cellular Organization
N-linked oligosaccharide is attaching carbohydrate chain to the amide nitrogen of which amino acid residue?
TLS Online TPP Program
#Question id: 2756
#Unit 2. Cellular Organization
The major protein components of chromatin referred as:
TLS Online TPP Program
#Question id: 10677
#Unit 10. Ecological Principles
What interactions occur in epiphyte plants that grow on other plants?
TLS Online TPP Program
#Question id: 13058
#Unit 13. Methods in Biology
Precision will be reduced, but yield will be increased
Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.
Which of the following true about real time PCR?