An experiment was done to see the effect of auxin: cytokinin ratios on morphogenesis can also be seen in crown gall tumors by mutation of the T-DNA of the Agrobacterium Ti plasmid,

I) Mutating the ipt gene (the tmr locus) of the Ti plasmid blocks zeatin biosynthesis in the infected cells

II) mutating either of the genes for auxin biosynthesis (tms locus)

Given some results about these above mutations ,

a) equal auxin to cytokinin ratio form undifferentiated callus tissue

b) high auxin:cytokinin ratio in the tumor cells causes the proliferation of roots instead of undifferentiated callus tissue

c) lowers the auxin:cytokinin ratio and stimulates the proliferation of shoots

What would be the result of these two mutation ?

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#Question id: 18661

#Unit 13. Methods in Biology

Which of the following highly sensitive analytical technique particularly useful for studying microheterogeneity in a protein

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#Question id: 18662

#Unit 13. Methods in Biology

Which of the following method is particularly useful for separating isoenzymes, which are different forms of the same enzyme, often differing by only one or two amino-acid residues.

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#Question id: 18663

#Unit 13. Methods in Biology

When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by

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#Question id: 18664

#Unit 13. Methods in Biology

2D electrophoresis is the combination of 

TLS Online TPP Program

#Question id: 18665

#Unit 13. Methods in Biology

In a road accident a forensic scientist collected traces of blood or other biological fluids and he wants to compared samples according to the composition of certain isoenzymes, for this which technique will efficiently work

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#Question id: 18666

#Unit 13. Methods in Biology

The above figure shows where sample applied was 100 µg of total protein extracted from a normal dog heart ventricle. The first dimension was conducted using a pH 4–7 isoelectric focussing gel. The second dimension was a 12% SDS–PAGE vertical slab gel. The pattern was visualised by silver staining. Which of the following techniques is applied