Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

#Question id: 3602

#Unit 8. Inheritance Biology

Mendel crossed Yellow, round and tall pea plants ( YYRRTT) with green, wrinkle and dwarf ones ( yyrrtt). The F1 plants were all yellow, round tall. These F1 plants were cross with YyrrTT. Which of the following proportional of phenotype in progeny?

#Question id: 3603

#Unit 8. Inheritance Biology

Blue follower is dominant over white flower and long pollen is dominant over short pollen. A plant with Blue flower and short pollen was cross with white flower and long pollen. Their progeny, ½ had a blue flower with long pollen and another ½ had a white flower with long pollen was allowed to intercross. Which of the following observation is correct among these progeny when independently assort?

#Question id: 3604

#Unit 8. Inheritance Biology

Genes A, B and C. control three phenotypes which assort independently; A plant with the genotype aaBbCc were cross with AabbCc. What is the probability for progeny which show the dominant phenotype for AT LEAST ONE of the phenotypes controlled by genes A, B and C?

#Question id: 3605

#Unit 8. Inheritance Biology

Male Plant blue flower with long pollen was crossed with female plant white flower with long pollen. Analysis of progeny of this cross showed that 50% of the progeny was red with long pollen while the remaining 50% of the progeny was white with long pollen. Which one of the following statements is a possible explanation of the above results?

#Question id: 3606

#Unit 8. Inheritance Biology

Genes A, B and C. control three phenotypes which assort independently; A plant with the genotype Aa Bb Cc is selfed. What is the probability for progeny which show the heterozygocity for AT LEAST ONE locus?

#Question id: 3607

#Unit 8. Inheritance Biology

Cystic fibrosis is caused by a recessive lethal gene and can be detected by an excess concentration of chloride in sweat. If a sweat test reveals a man to be heterozygous and his wife to be homozygous normal, what are the chances that their children will have the disease?