Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

#Question id: 10758

#Unit 6. System Physiology – Plant

The component of the soil water potential is hydrostatic pressure  (ψp) ,

#Question id: 10759

#Unit 6. System Physiology – Plant

many secondary metabolites have been suggested to have important ecological functions in plants:

a) they protect plants against being eaten by herbivores (herbivory)

b) they protect plants against being infected by microbial pathogens

c) they serve as attractants for pollinators and seed dispersing animals

d) they serve as agents of plant–plant competition

Which of the following combination from above statements is correct?

#Question id: 10760

#Unit 6. System Physiology – Plant

Following are some statements of secondary metabolites of terpenes in plants is incorrect?

#Question id: 10761

#Unit 6. System Physiology – Plant

Which one of the following combinations of secondary metabolite biosynthetic pathway found in the biosynthesis of terpenes?

#Question id: 10762

#Unit 6. System Physiology – Plant

The following are certain statements about secondary metabolites:

a) They protect plants against being eaten by herbivores and against being infected by microbial pathogens

b) Terpenes, are the class of secondary metabolites are synthesized by methyl erythritol phosphate(MEP) pathway and Mevalonic acid pathway

c) Terpenes, are the class of secondary metabolites are synthesized by methyl erythritol phosphate(MEP) pathway and sikkimic acid pathway

Which one of the following combinations of the above statements is correct?

#Question id: 10763

#Unit 6. System Physiology – Plant

In mevalonic acid pathway, three molecules of acetyl-CoA are joined together stepwise to form mevalonic acid, which intermediate steps it will perform to yield isopentenyl diphosphate (IPP2)?