Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

How primer concentration leads to decrease in PCR efficiency?

TLS Online TPP Program

#Question id: 1596

#Unit 4. Cell Communication and Cell Signaling

Which one of the following is function of Class III MHC genes?

TLS Online TPP Program

#Question id: 14904

#Unit 6. System Physiology – Plant

Cell walls commonly are classified into two major types. This classification is based not on structural or biochemical differences;

A) primary walls and

B) secondary walls

i) walls formed during cell growth

ii) walls that are formed after cell enlargement stops

iii) multilayered walls that are strengthened and waterproofed by lignin

iv) Usually they are thin and simple, but sometimes may be thick and multilayered, such as those found in collenchyma or in the epidermis

v) highly specialized in structure and composition, reflecting the differentiated state of the cell

Which of the following correctly represents features of primary and secondary walls?

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#Question id: 63

#Unit 1. Molecules and their Interaction Relevant to Biology

The two molecules shown in the figures are best described as ________.

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#Question id: 3494

#Unit 11. Evolution and Behavior

Which of the following is a disadvantage of group living for many species?

a. Competition between individuals for resources is increased

b. It can reduce predator success

c. It can reduce reproductive success

d. It increased transmission of parasites and other diseases

TLS Online TPP Program

#Question id: 20303

#Unit 7. System Physiology – Animal

Which of the following correctly represents the physiological effect of adrenalin?