Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Efficiency during ligation of blunt ended insert and vector cannot be cannot be enhanced by means of…..

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#Question id: 1275

#Unit 4. Cell Communication and Cell Signaling

The functions of the extracellular matrix include:

a. supporting differentiation.

b. inducing morphogenesis.

c. binding growth hormones.

d. repairing damaged tissue

which of the following combination is correct?

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#Question id: 28347

#Unit 5. Developmental Biology

Par-1 protein is responsible for___

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#Question id: 26587

#Unit 3. Fundamental Processes

Circularisation of telomere known as

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#Question id: 23146

#Unit 13. Methods in Biology

A phenomenon of absorption of light at a certain wavelength and then emission at longer wavelength is known as

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#Question id: 26902

#Unit 10. Ecological Principles

Which characteristics are considered the species that are more prone to extinction?