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TLS Online TPP Program

#Question id: 3855

In eukaryotes, which of the following modifications is made to a primary transcript prior to its transport out of the nucleus?

#Biochemistry

  1. poly(A) tails added to the 3' end 

  2. 7-methyl guanosine caps added to the 5' end

  3. exon sequences, when present, are spliced out 

  4. all of these

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TLS Online TPP Program

#Question id: 39280

#Plant Biotechnology

Q. Regarding cybridization for male sterile line development:
Statement I: Cybrids allow rapid transfer of mitochondria even across sexually incompatible species.
Statement II: Male sterile lines developed via protoplast fusion can be used for hybrid seed production using CMS restorers.
Statement III: Nuclear genomes are exchanged between donor and recipient protoplasts in cybridization.
Which statements are correct?
TLS Online TPP Program

#Question id: 39281

#Plant Biotechnology

Q. Statement I: Conventional backcrossing is a more time-efficient method for cytoplasm transfer compared to somatic hybridization. 
Statement II: Protoplast fusion can achieve cytoplasm transfer in approximately one year.
TLS Online TPP Program

#Question id: 39282

#Plant Biotechnology

Q. Statement I: In the cybrid approach, iodoacetate is used to inactivate the nuclei of the recipient strain (Parent A). 
Statement II: Irradiation is used to inactivate the nuclei of the donor strain (Parent B) to ensure only its cytoplasm is transferred.
TLS Online TPP Program

#Question id: 39283

#Plant Biotechnology

Q. Statement I: In the fusion product described, the mitochondria are always derived from the donor strain (Parent B).
 Statement II: The chloroplasts in the resulting regenerants must always come from the recipient strain (Parent A)
TLS Online TPP Program

#Question id: 39284

#Plant Biotechnology

Q. Statement I: Fusing tomato protoplasts with irradiated Solanum acaule protoplasts produced plants that were morphologically identical to Solanum acaule. 
Statement II: The resulting tomato regenerants exhibited Cytoplasmic Male Sterility (CMS), which was inherited maternally.
TLS Online TPP Program

#Question id: 39285

#Plant Biotechnology

Q. Statement I: Protoplast fusion can be used to develop male sterile lines even between sexually incompatible species. 
Statement II: CMS lines developed through this method cannot be used with traditional CMS restorers.