TLS Online TPP Program

#Question id: 2942


During mitosis, the breakdown of the nuclear envelope depends on the:

#Part-A Aptitude & General Biotechnology
  1. disassembly following proteasomal degradation of intermediate filaments.

  2. disassembly of lamin filaments following phosphorylation by mitotic cyclin/cdk complexes.

  3. ubiquitination of mitotic cyclin proteins.

  4. lamin filament dephosphorylation by cdc14.

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TLS Online TPP Program

#Question id: 18955

#Part-A Aptitude & General Biotechnology

(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of

TLS Online TPP Program

#Question id: 18957

#Part-A Aptitude & General Biotechnology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are

TLS Online TPP Program

#Question id: 18958

#Part-A Aptitude & General Biotechnology

The number of clones to represent this fragment in genomic library with a probability of 95% are

TLS Online TPP Program

#Question id: 18959

#Part-A Aptitude & General Biotechnology

 Vectors used for construction of genomic libraries, and their capacity is given match them correctly
A- λ EMBL series                                                                                   I - 300kb - 1,400 kb
B - Cosmids                                                                                                 II - 45 kb
C – BACs and YACs (high capacity vectors)                                             III - 20kb

TLS Online TPP Program

#Question id: 18960

#Part-A Aptitude & General Biotechnology

cDNA library do not require very large vectors hence which of the following is commonly used in cDNA library

TLS Online TPP Program

#Question id: 18961

#Part-A Aptitude & General Biotechnology

When a cDNA library is generated by first removing those mRNA or cDNA sequences that are common to two sources, e.g., two different Cell types, such a library is called