TLS Online TPP Program

#Question id: 4361


How do steroid hormones produce their effects in cells?

#Part-A Aptitude & General Biotechnology
  1. by binding to the regulatory gene in an operon

  2. by activating translation of certain mRNAs

  3. by promoting the degradation of specific mRNAs

  4. by binding to intracellular receptors and promoting transcription of specific genes

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TLS Online TPP Program

#Question id: 19884

#Part-A Aptitude & General Biotechnology

The first successful transformation of rDNA molecules into a bacterium was carried out by

TLS Online TPP Program

#Question id: 19885

#Part-A Aptitude & General Biotechnology

The plasmid used by Cohen and Boyer fr their transformation experiment was

TLS Online TPP Program

#Question id: 19886

#Part-A Aptitude & General Biotechnology

Gene cloning refers to the
I) Production of large number of copies of the gene being cloned
II) Production of asexual progeny from a single individual or a cell 
III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

TLS Online TPP Program

#Question id: 19887

#Part-A Aptitude & General Biotechnology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Part-A Aptitude & General Biotechnology

There are some typical enzyme used in the manipulation of nucleic acid;

                 

                 ENZYMES

 

USE IN NUCLEIC ACID MANIPULATION

 

A) klenow

 

i) DNA-dependent RNA polymerase

 

B) T4 DNA polymerase

 

ii) Single-strand specific nuclease

 

C) Taq DNA polymerase

 

iii) DNA pol I lacks 5’→ 3’ exonuclease activity

 

D) T7 RNA polymerase

 

iv) Thermostable DNA polymerase used in PCR

 

E) S1 nuclease

 

v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19929

#Part-B Specialized Branches in Biotechnology

he growth of an organism on glucose is described by the following Monod model parameters: μm = 0.5 h-1 and Ks =0.1 g.l-1, if the concentration of glucose in the feed is 10 g.l-1 and the dilution rate is set to 0.4 h-1, then the steady state concentration of glucose in the effluent will be