If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?

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#Question id: 5730

#Part-B Specialized Branches in Biotechnology

In a family, father is homozygous dominant (AA) for a gene A and his wife is homozygous for its ressive allele (aa) showing albino phenotype. It was surprising that their child showed the albino phenotype. Which of the following phenomenon can explain the phenotype?

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#Question id: 2089

#Part-A Aptitude & General Biotechnology

What type of single-pass membrane protein would contain a hydrophobic membrane spanning alpha-helix?

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#Question id: 424

#Part-A Aptitude & General Biotechnology

Phosphorylation at the expense of ATP is catalyzed by ________.

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#Question id: 13061

#Part-A Aptitude & General Biotechnology

Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

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#Question id: 1561

#Part-B Specialized Branches in Biotechnology

The cells and signaling molecules involved in the initial stages of the inflammatory response are ?