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TLS Online TPP Program

#Question id: 887

Polypeptides fold rapidly by a stepwise process, how does the polypeptide chain arrive at its native conformation?

#Part-B Specialized Branches in Biotechnology

  1. proteins appear to fold in a hierarchical manner, with small local elements of structure forming and then to yield larger elements, which coalesce with other such elements to form yet larger elements and converted into its native conformation

  2. A protein-folding pathway has been highly successful in predicting the three-dimensional structure of small proteins from their amino acid sequence atleast 156 amino acid residue that have acquired their final structure

  3. Vander waal interaction play a significant role throughout the process, as the aggregation of nonpolar amino acid side chains provides an entropic stabilization to intermediates and, eventually, to the final folded structure

  4. The native conformation of the protein folding by stepwise manner such as unfolded→transition state→molten globule→folded conformation by decreasing the free energy

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TLS Online TPP Program

#Question id: 14255

#Part-B Specialized Branches in Biotechnology

Hybridoma cells immobilized on surfaces of Sephadex beads are used in a packed column for production of monoclonoal antibodies (Mab). Hybridoma concentration is approximately X = 5 g/l in the bed. The flow rate of the synthetic medium and glucose concentration are Q = 2 l/h and S0 = 40 g/l, respectively. The rate constant for Mab formation is k = 1 gX/l-d. Assume that there are no diffusion limitations and glucose is the rate limiting nutrient.  If Yp/s is 4 mg Mab/g glu, determine the effluent Mab concentration of the system?
TLS Online TPP Program

#Question id: 14796

#Part-A Aptitude & General Biotechnology

Interception is a type of filter sterilization, which is used for the removal of 
TLS Online TPP Program

#Question id: 5255

#Part-A Aptitude & General Biotechnology

Which of the following is a structure that permits conjugation to occur?
TLS Online TPP Program

#Question id: 5946

#Part-A Aptitude & General Biotechnology

Today is Sunday. After 61 days it was?
TLS Online TPP Program

#Question id: 2641

#Part-A Aptitude & General Biotechnology

Which segments of the attenuator together form the repression loop?