Which of the following statement is true?

A) PAM matrices are derived from an evolutionary model whereas the BLOSUM matrices consist of entirely direct observations. 

B) Because of BLOSUM matrices have less evolutionary meaning that is why the PAM matrices are used most often for reconstructing phylogenetic trees.

C) BLOSUM matrices are entirely derived from local sequence alignments of conserved sequence blocks, whereas the PAM matrix is based on the global alignment of full-length sequences.

D) BLOSUM matrices are more advantageous in searching databases and finding conserved domains in proteins.

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#Question id: 18773

#Part-A Aptitude & General Biotechnology

The affinity constant, Ka between antigen-antibodies, can be determined by 

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#Question id: 18774

#Part-A Aptitude & General Biotechnology

The above Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. In these plots, r equals moles of bound ligand/mole antibody and c is the concentration of free ligand, (Ka) is slope while intercept on x axis represent 

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#Question id: 18775

#Part-A Aptitude & General Biotechnology

which of the following technique is a highly sensitive and quantitative procedure that utilizes radioactively labelled antigen or antibody

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#Question id: 18776

#Part-A Aptitude & General Biotechnology

In Western blotting, a protein mixture is separated by electrophoresis; then the protein bands are electrophoretically transferred onto nitrocellulose and identified with 

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#Question id: 18777

#Part-A Aptitude & General Biotechnology

which of the following technique using antibodies labelled with fluorescent molecules can be used to visualize antigen on or within cells.

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#Question id: 18778

#Part-A Aptitude & General Biotechnology

which of the following technique provides an unusually powerful technology for the quantitative analysis and sorting of cell populations labelled with one or more fluorescent antibodies.