Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Which of the following will provide least specific amplification in qPCR?

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#Question id: 343

#Part-B Specialized Branches in Biotechnology

Assume that acid rain has lowered the pH of a particular lake to pH 5.0. What is the hydroxide ion concentration of this lake?

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#Question id: 18907

#Part-A Aptitude & General Biotechnology

When expression of the DNA insert is desired, the vector should contain at least suitable control elements, 

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#Question id: 658

#Part-A Aptitude & General Biotechnology

To what level of structure do α-helices belong?

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#Question id: 3016

#Part-A Aptitude & General Biotechnology

Prokaryotic cells divide by a process known as

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#Question id: 10881

#Part-B Specialized Branches in Biotechnology

In deep water, which of the following abiotic factors would most limit primary productivity?