Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Arrange in the order of their increasing capacity:

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#Question id: 18944

#Part-A Aptitude & General Biotechnology

A Linear or circular shuttle vector; behave in yeast like chromosomes; contain, ARS, CEN and telomeric sequences; used for cloning up to 1000 kb DNA is

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#Question id: 4327

#Part-A Aptitude & General Biotechnology

What would occur if the repressor of an inducible operon were mutated so it could not bind the operator?

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#Question id: 11873

#Part-A Aptitude & General Biotechnology

A, B and C start running at the same time and at the same point in the same direction in a circular stadium. A completes a round in 252 seconds, B in 308 seconds and C in 198 seconds. After what time will they meet again at the starting point?

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#Question id: 4804

#Part-A Aptitude & General Biotechnology

Formation of the following pattern in an ordered octad is due to what event during meiosis? Pattern: AAaaaaaAA

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#Question id: 18649

#Part-A Aptitude & General Biotechnology

In an SDS PAGE, on average, one SDS molecule binds for every