Precision will be reduced, but yield will be increased

Optimisation of a PCR reaction is often a compromise between the competing demands for precision, efficiency and yield. Although the specific effects may vary, generally, increasing the annealing temperature will increase non-specific primer binding and reduce precision. Increasing the length of the elongation phase will reduce the proportion of incomplete newly-synthesised strands and therefore increase yield. In this case, the potential effect on efficiency is unclear. Increasing the elongation phase would increase the reaction time, but the time taken to ramp down to a lower annealing temperature would be reduced.

Agglutination of antigens by utilising specific antibodies can only occur, if

TLS Online TPP Program

#Question id: 19883

#Part-A Aptitude & General Biotechnology

Which of the following statements are true regarding rDNA technology?

a) rDNA technology is used to obtain large number of copies of specific DNA fragments

b) rDNA technology is used to obtain large quantities of the protein produced by the concerned gene

c) rDNA technology is used to integrate gene of interest into chromosomes where it express itself

TLS Online TPP Program

#Question id: 19884

#Part-A Aptitude & General Biotechnology

The first successful transformation of rDNA molecules into a bacterium was carried out by

TLS Online TPP Program

#Question id: 19885

#Part-A Aptitude & General Biotechnology

The plasmid used by Cohen and Boyer fr their transformation experiment was

TLS Online TPP Program

#Question id: 19886

#Part-A Aptitude & General Biotechnology

Gene cloning refers to the

I) Production of large number of copies of the gene being cloned

II) Production of asexual progeny from a single individual or a cell 

III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

TLS Online TPP Program

#Question id: 19887

#Part-A Aptitude & General Biotechnology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Part-A Aptitude & General Biotechnology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity