G1/S Cyclin–CDKs

In human - cyclin E-CDK 2 

In budding yeast - CIn 1/2-Cdc 28

TLS Online TPP Program

#Id: 8869

#Section 3: Genetics, Cellular and Molecular Biology

Yeast cells with a defective wee1+ gene activate mitotic CDKs prematurely and premature entry into mitosis (smaller than normal cells

TLS Online TPP Program

#Id: 8870

#Section 3: Genetics, Cellular and Molecular Biology

Fission yeast cells carrying a CDK1 mutation in which the tyrosine-15 residue is replaced by phenylalanine

show the same premature mitotic CDK activation and entry into mitosis.

TLS Online TPP Program

#Id: 8871

#Section 3: Genetics, Cellular and Molecular Biology

Until Mitotic CDKs is not active, the activatory protein phosphatase, Cdc25, is phosphorylated, bound and inhibited by phosphoserine/threonine-binding protein 14-3-3.

TLS Online TPP Program

#Id: 8872

#Section 3: Genetics, Cellular and Molecular Biology

The entire process of mitotic CDKs activation and entry into mitosis involves two positive feedback loops and coordination between events at the centrosome, in the cytoplasm, and in the nucleus.

TLS Online TPP Program

#Id: 8873

#Section 3: Genetics, Cellular and Molecular Biology

Cdc25B, activating an initial small pool of cyclin B/1 at the centrosome, which is facilitate centrosome maturation.

TLS Online TPP Program

#Id: 8874

#Section 3: Genetics, Cellular and Molecular Biology

Initial pool of active cyclin B-CDK1 phosphorylates Cdc25C, this recruits Pin1 and Plk1, which recognize Cdc25C through their phospho-binding domains.