Direct submission to GenBank are made using 

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#Question id: 5374

#General Aptitude

The average of 11 result is 50 . if the average of first 6 result is 49 and that of last 6 results is 52,find the sixth result .

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#Question id: 10265

#Section 3: Genetics, Cellular and Molecular Biology

The alternate form of a gene is

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#Question id: 2004

#Section 2: General Biology

A bone marrow transplant may not be appropriate from a given donor (Jane) to a given recipient (Janeʹs cousin Bob), even though Jane has previously given blood for one of Bobʹs needed transfusions. Which of the following might account for this?

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#Question id: 13088

#Section 7: Recombinant DNA technology and Other Tools in Biotechnology

To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.

 

The distance between each tick mark placed on the line above is 100 bases in length

Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’

 

The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.

                              

You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.

 

What is the Colony 1’s plasmid is;

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#Question id: 1560

#Section 2: General Biology

An invertebrate, such as an insect, has innate immunity that can be nonspecific about which pathogens are prevented from harming its metabolism. Which of the following is most likely to function this way in the insectʹs intestine?